Construct: shRNA TRCN0000148013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181840.1-466s1c1
DNA Barcode:
GCAACCATCTTATCTACATCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNK18 (338567)
Gene Description:
potassium two pore domain channel subfamily K member 18
Transcript:
RefSeq NM_181840.1 (CURRENT)
Match location:
Position 466 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACCATCTTATCTACATCT
Hairpin Sequence:
5'-CCGG-GCAACCATCTTATCTACATCT-CTCGAG-AGATGTAGATAAGATGGTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACCATCTTATCTACATCTCTCGAGAGATGTAGATAAGATGGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACCATCTTATCTACATCTCTCGAGAGATGTAGATAAGATGGTTGC-3'

Other clones with same target sequence:

(none)