Construct: shRNA TRCN0000156225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199181.2-3030s1c1
DNA Barcode:
CAGTGAGAAGATCCACGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SOGA1 (140710)
Gene Description:
suppressor of glucose, autophagy associated 1
Transcript:
RefSeq NM_199181.2 (CURRENT)
Match location:
Position 3030 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGAGAAGATCCACGACAA
Hairpin Sequence:
5'-CCGG-CAGTGAGAAGATCCACGACAA-CTCGAG-TTGTCGTGGATCTTCTCACTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGAGAAGATCCACGACAACTCGAGTTGTCGTGGATCTTCTCACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGAGAAGATCCACGACAACTCGAGTTGTCGTGGATCTTCTCACTG-3'

Other clones with same target sequence:

(none)