Construct: shRNA TRCN0000157005

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020433.4-4409s1c1
DNA Barcode:
GCTTCACACTTGCCGTTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JPH2 (57158)
Gene Description:
junctophilin 2
Transcript:
RefSeq NM_020433.4 (CURRENT)
Match location:
Position 4409 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157005)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCACACTTGCCGTTTCAA
Hairpin Sequence:
5'-CCGG-GCTTCACACTTGCCGTTTCAA-CTCGAG-TTGAAACGGCAAGTGTGAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCACACTTGCCGTTTCAACTCGAGTTGAAACGGCAAGTGTGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCACACTTGCCGTTTCAACTCGAGTTGAAACGGCAAGTGTGAAGC-3'

Other clones with same target sequence:

(none)