Construct: shRNA TRCN0000160083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032831.1-978s1c1
DNA Barcode:
CCTCTTGTCTTAATACCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ORAI2 (80228)
Gene Description:
ORAI calcium release-activated calcium modulator 2
Transcript:
RefSeq NM_032831.1 (NON-CURRENT)
Match location:
Position 978 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000160083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTGTCTTAATACCATAA
Hairpin Sequence:
5'-CCGG-CCTCTTGTCTTAATACCATAA-CTCGAG-TTATGGTATTAAGACAAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTGTCTTAATACCATAACTCGAGTTATGGTATTAAGACAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTGTCTTAATACCATAACTCGAGTTATGGTATTAAGACAAGAGG-3'

Other clones with same target sequence:

(none)