Construct: shRNA TRCN0000163173

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015264.1-917s1c1
DNA Barcode:
GAGTGTCTACAGGTGACACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIAA0930 (23313)
Gene Description:
KIAA0930
Transcript:
RefSeq NM_015264.1 (CURRENT)
Match location:
Position 917 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163173)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGTCTACAGGTGACACAT
Hairpin Sequence:
5'-CCGG-GAGTGTCTACAGGTGACACAT-CTCGAG-ATGTGTCACCTGTAGACACTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGTCTACAGGTGACACATCTCGAGATGTGTCACCTGTAGACACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGTCTACAGGTGACACATCTCGAGATGTGTCACCTGTAGACACTC-3'

Other clones with same target sequence:

(none)