Construct: shRNA TRCN0000168060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182481.1-759s1c1
DNA Barcode:
CGGCTGGAAATGCAAATGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAGE3 (85318)
Gene Description:
BAGE family member 3
Transcript:
RefSeq NM_182481.1 (CURRENT)
Match location:
Position 759 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000168060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTGGAAATGCAAATGAAT
Hairpin Sequence:
5'-CCGG-CGGCTGGAAATGCAAATGAAT-CTCGAG-ATTCATTTGCATTTCCAGCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTGGAAATGCAAATGAATCTCGAGATTCATTTGCATTTCCAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTGGAAATGCAAATGAATCTCGAGATTCATTTGCATTTCCAGCCG-3'

Other clones with same target sequence:

(none)