Construct: shRNA TRCN0000172943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001187.1-767s1c1
DNA Barcode:
GAGGGATCATTGCCAGAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAGE (574)
Gene Description:
B melanoma antigen
Transcript:
RefSeq NM_001187.1 (CURRENT)
Match location:
Position 767 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000172943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGGATCATTGCCAGAGATT
Hairpin Sequence:
5'-CCGG-GAGGGATCATTGCCAGAGATT-CTCGAG-AATCTCTGGCAATGATCCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGGATCATTGCCAGAGATTCTCGAGAATCTCTGGCAATGATCCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGGATCATTGCCAGAGATTCTCGAGAATCTCTGGCAATGATCCCTC-3'

Other clones with same target sequence:

(none)