Construct: shRNA TRCN0000173257

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134138.1-894s1c1
DNA Barcode:
CTCATCCTGCTGAGATCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psmg2 (107047)
Gene Description:
proteasome (prosome, macropain) assembly chaperone 2
Transcript:
RefSeq NM_134138.1 (CURRENT)
Match location:
Position 894 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173257)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATCCTGCTGAGATCACAA
Hairpin Sequence:
5'-CCGG-CTCATCCTGCTGAGATCACAA-CTCGAG-TTGTGATCTCAGCAGGATGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATCCTGCTGAGATCACAACTCGAGTTGTGATCTCAGCAGGATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATCCTGCTGAGATCACAACTCGAGTTGTGATCTCAGCAGGATGAG-3'

Other clones with same target sequence:

  1. TRCN0000350140