Construct: shRNA TRCN0000173763

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207535.1-417s1c1
DNA Barcode:
CGTGTTGATCTGCATTCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrgpra7 (404236)
Gene Description:
MAS-related GPR, member A7
Transcript:
RefSeq NM_207535.1 (CURRENT)
Match location:
Position 417 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173763)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTTGATCTGCATTCTGAA
Hairpin Sequence:
5'-CCGG-CGTGTTGATCTGCATTCTGAA-CTCGAG-TTCAGAATGCAGATCAACACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTTGATCTGCATTCTGAACTCGAGTTCAGAATGCAGATCAACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTTGATCTGCATTCTGAACTCGAGTTCAGAATGCAGATCAACACG-3'

Other clones with same target sequence:

(none)