Construct: shRNA TRCN0000173803

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025696.1-2545s1c1
DNA Barcode:
CCGGAGGATTGTATCCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sorcs3 (66673)
Gene Description:
sortilin-related VPS10 domain containing receptor 3
Transcript:
RefSeq NM_025696.1 (NON-CURRENT)
Match location:
Position 2545 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000173803)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGGATTGTATCCAACAA
Hairpin Sequence:
5'-CCGG-CCGGAGGATTGTATCCAACAA-CTCGAG-TTGTTGGATACAATCCTCCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGGATTGTATCCAACAACTCGAGTTGTTGGATACAATCCTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGGATTGTATCCAACAACTCGAGTTGTTGGATACAATCCTCCGG-3'

Other clones with same target sequence:

(none)