Construct: shRNA TRCN0000174034

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198425.2-1125s1c1
DNA Barcode:
CGAGGTTTCTGTGGAGAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eid2 (386655)
Gene Description:
EP300 interacting inhibitor of differentiation 2
Transcript:
RefSeq NM_198425.2 (CURRENT)
Match location:
Position 1125 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174034)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGTTTCTGTGGAGAACTT
Hairpin Sequence:
5'-CCGG-CGAGGTTTCTGTGGAGAACTT-CTCGAG-AAGTTCTCCACAGAAACCTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGTTTCTGTGGAGAACTTCTCGAGAAGTTCTCCACAGAAACCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGTTTCTGTGGAGAACTTCTCGAGAAGTTCTCCACAGAAACCTCG-3'

Other clones with same target sequence:

(none)