Construct: shRNA TRCN0000174236

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031857.1-1835s1c2
DNA Barcode:
CTGATTAGTGTGATCGACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHA9 (9752)
Gene Description:
protocadherin alpha 9
Transcript:
RefSeq NM_031857.1 (CURRENT)
Match location:
Position 1835 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000174236)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGATTAGTGTGATCGACCTA
Hairpin Sequence:
5'-CCGG-CTGATTAGTGTGATCGACCTA-CTCGAG-TAGGTCGATCACACTAATCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGATTAGTGTGATCGACCTACTCGAGTAGGTCGATCACACTAATCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGATTAGTGTGATCGACCTACTCGAGTAGGTCGATCACACTAATCAG-3'

Other clones with same target sequence:

  1. TRCN0000053276