Construct: shRNA TRCN0000175107

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144793.1-429s1c1
DNA Barcode:
GTATTCTTCGAAGCAGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc25a38 (208638)
Gene Description:
solute carrier family 25, member 38
Transcript:
RefSeq NM_144793.1 (CURRENT)
Match location:
Position 429 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175107)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATTCTTCGAAGCAGTATTT
Hairpin Sequence:
5'-CCGG-GTATTCTTCGAAGCAGTATTT-CTCGAG-AAATACTGCTTCGAAGAATAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATTCTTCGAAGCAGTATTTCTCGAGAAATACTGCTTCGAAGAATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATTCTTCGAAGCAGTATTTCTCGAGAAATACTGCTTCGAAGAATAC-3'

Other clones with same target sequence:

(none)