Construct: shRNA TRCN0000175183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134200.1-540s1c1
DNA Barcode:
CAATGCAGTATTACTCTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r237 (171234)
Gene Description:
vomeronasal 1 receptor 237
Transcript:
RefSeq NM_134200.1 (CURRENT)
Match location:
Position 540 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATGCAGTATTACTCTCTAT
Hairpin Sequence:
5'-CCGG-CAATGCAGTATTACTCTCTAT-CTCGAG-ATAGAGAGTAATACTGCATTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATGCAGTATTACTCTCTATCTCGAGATAGAGAGTAATACTGCATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATGCAGTATTACTCTCTATCTCGAGATAGAGAGTAATACTGCATTG-3'

Other clones with same target sequence:

(none)