Construct: shRNA TRCN0000176188

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004149.1-2352s1c1
DNA Barcode:
GCTTTCCGTAAGAACTGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp366 (238803)
Gene Description:
zinc finger protein 366
Transcript:
RefSeq NM_001004149.1 (CURRENT)
Match location:
Position 2352 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176188)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCCGTAAGAACTGTCAT
Hairpin Sequence:
5'-CCGG-GCTTTCCGTAAGAACTGTCAT-CTCGAG-ATGACAGTTCTTACGGAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCCGTAAGAACTGTCATCTCGAGATGACAGTTCTTACGGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCCGTAAGAACTGTCATCTCGAGATGACAGTTCTTACGGAAAGC-3'

Other clones with same target sequence:

(none)