Construct: shRNA TRCN0000176340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198425.2-953s1c1
DNA Barcode:
CCTACCGTTTCTTGAGTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eid2 (386655)
Gene Description:
EP300 interacting inhibitor of differentiation 2
Transcript:
RefSeq NM_198425.2 (CURRENT)
Match location:
Position 953 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACCGTTTCTTGAGTTTAT
Hairpin Sequence:
5'-CCGG-CCTACCGTTTCTTGAGTTTAT-CTCGAG-ATAAACTCAAGAAACGGTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACCGTTTCTTGAGTTTATCTCGAGATAAACTCAAGAAACGGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACCGTTTCTTGAGTTTATCTCGAGATAAACTCAAGAAACGGTAGG-3'

Other clones with same target sequence:

(none)