Construct: shRNA TRCN0000176756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172916.2-13662s1c1
DNA Barcode:
CCTAAAGAAGTCTGTAAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hydin (244653)
Gene Description:
HYDIN, axonemal central pair apparatus protein
Transcript:
RefSeq NM_172916.2 (CURRENT)
Match location:
Position 13662 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAAGAAGTCTGTAAACTA
Hairpin Sequence:
5'-CCGG-CCTAAAGAAGTCTGTAAACTA-CTCGAG-TAGTTTACAGACTTCTTTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAAGAAGTCTGTAAACTACTCGAGTAGTTTACAGACTTCTTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAAGAAGTCTGTAAACTACTCGAGTAGTTTACAGACTTCTTTAGG-3'

Other clones with same target sequence:

(none)