Construct: shRNA TRCN0000176876

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170779.1-205s1c1
DNA Barcode:
GATTACTTCATAGACCACAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wwc1 (211652)
Gene Description:
WW, C2 and coiled-coil domain containing 1
Transcript:
RefSeq NM_170779.1 (CURRENT)
Match location:
Position 205 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176876)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTACTTCATAGACCACAAT
Hairpin Sequence:
5'-CCGG-GATTACTTCATAGACCACAAT-CTCGAG-ATTGTGGTCTATGAAGTAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTACTTCATAGACCACAATCTCGAGATTGTGGTCTATGAAGTAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTACTTCATAGACCACAATCTCGAGATTGTGGTCTATGAAGTAATC-3'

Other clones with same target sequence:

  1. TRCN0000341160