Construct: shRNA TRCN0000177187

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198019.2-2418s1c1
DNA Barcode:
GTGATACTCTTGGATCTGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cep78 (208518)
Gene Description:
centrosomal protein 78
Transcript:
RefSeq NM_198019.2 (CURRENT)
Match location:
Position 2418 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177187)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGATACTCTTGGATCTGATT
Hairpin Sequence:
5'-CCGG-GTGATACTCTTGGATCTGATT-CTCGAG-AATCAGATCCAAGAGTATCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGATACTCTTGGATCTGATTCTCGAGAATCAGATCCAAGAGTATCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGATACTCTTGGATCTGATTCTCGAGAATCAGATCCAAGAGTATCAC-3'

Other clones with same target sequence:

(none)