Construct: shRNA TRCN0000177862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153542.1-266s1c1
DNA Barcode:
CTCTGATCAGATCCATCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrc20 (216011)
Gene Description:
leucine rich repeat containing 20
Transcript:
RefSeq NM_153542.1 (CURRENT)
Match location:
Position 266 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTGATCAGATCCATCTCAT
Hairpin Sequence:
5'-CCGG-CTCTGATCAGATCCATCTCAT-CTCGAG-ATGAGATGGATCTGATCAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTGATCAGATCCATCTCATCTCGAGATGAGATGGATCTGATCAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTGATCAGATCCATCTCATCTCGAGATGAGATGGATCTGATCAGAG-3'

Other clones with same target sequence:

(none)