Construct: shRNA TRCN0000178136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019989.2-255s1c1
DNA Barcode:
GCTTTGTTTCTTAGAAGCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh3bgrl (56726)
Gene Description:
SH3-binding domain glutamic acid-rich protein like
Transcript:
RefSeq NM_019989.2 (CURRENT)
Match location:
Position 255 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGTTTCTTAGAAGCCAA
Hairpin Sequence:
5'-CCGG-GCTTTGTTTCTTAGAAGCCAA-CTCGAG-TTGGCTTCTAAGAAACAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGTTTCTTAGAAGCCAACTCGAGTTGGCTTCTAAGAAACAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGTTTCTTAGAAGCCAACTCGAGTTGGCTTCTAAGAAACAAAGC-3'

Other clones with same target sequence:

(none)