Transcript: Mouse NM_019989.2

Mus musculus SH3-binding domain glutamic acid-rich protein like (Sh3bgrl), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Sh3bgrl (56726)
Length:
2856
CDS:
187..531

Additional Resources:

NCBI RefSeq record:
NM_019989.2
NBCI Gene record:
Sh3bgrl (56726)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_019989.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000337958 TTCTGGCTCTACGGCGATTAA pLKO_005 216 CDS 100% 13.200 10.560 N Sh3bgrl n/a
2 TRCN0000337991 GACAGCACCACCTGGTTCAAA pLKO_005 477 CDS 100% 5.625 4.500 N Sh3bgrl n/a
3 TRCN0000337928 CGCATGCAAACAAGCATAATG pLKO_005 659 3UTR 100% 13.200 9.240 N Sh3bgrl n/a
4 TRCN0000181391 CCTCAGTACAATGTGGGTATA pLKO.1 1266 3UTR 100% 10.800 7.560 N Sh3bgrl n/a
5 TRCN0000198019 CGTGAATGATACTGTCCTTAA pLKO.1 1467 3UTR 100% 10.800 7.560 N Sh3bgrl n/a
6 TRCN0000337927 GCAAATCAGCAAGCATGAATG pLKO_005 514 CDS 100% 10.800 7.560 N Sh3bgrl n/a
7 TRCN0000337930 GGGATTACGATGCCTTCTTTG pLKO_005 416 CDS 100% 10.800 7.560 N Sh3bgrl n/a
8 TRCN0000177530 CAAGATGTGCTTTGTTTCTTA pLKO.1 247 CDS 100% 5.625 3.938 N Sh3bgrl n/a
9 TRCN0000198465 GATTACGATGCCTTCTTTGAA pLKO.1 418 CDS 100% 5.625 3.938 N Sh3bgrl n/a
10 TRCN0000129485 GAAGAGAATCGGAAGTGGATG pLKO.1 313 CDS 100% 4.050 2.835 N SH3BGRL n/a
11 TRCN0000344880 GAAGAGAATCGGAAGTGGATG pLKO_005 313 CDS 100% 4.050 2.835 N SH3BGRL n/a
12 TRCN0000176923 GATTAAGAAGAAACAGCAAGA pLKO.1 231 CDS 100% 4.050 2.835 N Sh3bgrl n/a
13 TRCN0000182457 GAATCGGAAGTGGATGAGAGA pLKO.1 318 CDS 100% 2.640 1.848 N Sh3bgrl n/a
14 TRCN0000178136 GCTTTGTTTCTTAGAAGCCAA pLKO.1 255 CDS 100% 2.640 1.848 N Sh3bgrl n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019989.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01529 pDONR223 100% 92.6% 94.7% None (many diffs) n/a
2 ccsbBroad304_01529 pLX_304 0% 92.6% 94.7% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000465739 TTTGCAAGGTCCGTGACCGAGTTC pLX_317 98% 92.6% 94.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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