Construct: shRNA TRCN0000178723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198647.1-1604s1c1
DNA Barcode:
CTACAGACTCAAGTACATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbc1d22b (381085)
Gene Description:
TBC1 domain family, member 22B
Transcript:
RefSeq NM_198647.1 (CURRENT)
Match location:
Position 1604 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACAGACTCAAGTACATGTT
Hairpin Sequence:
5'-CCGG-CTACAGACTCAAGTACATGTT-CTCGAG-AACATGTACTTGAGTCTGTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACAGACTCAAGTACATGTTCTCGAGAACATGTACTTGAGTCTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACAGACTCAAGTACATGTTCTCGAGAACATGTACTTGAGTCTGTAG-3'

Other clones with same target sequence:

(none)