Construct: shRNA TRCN0000178756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207202.2-2507s1c1
DNA Barcode:
CCACCTCTTTAACAGCTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc120 (54648)
Gene Description:
coiled-coil domain containing 120
Transcript:
RefSeq NM_207202.2 (CURRENT)
Match location:
Position 2507 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTCTTTAACAGCTTCTT
Hairpin Sequence:
5'-CCGG-CCACCTCTTTAACAGCTTCTT-CTCGAG-AAGAAGCTGTTAAAGAGGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTCTTTAACAGCTTCTTCTCGAGAAGAAGCTGTTAAAGAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTCTTTAACAGCTTCTTCTCGAGAAGAAGCTGTTAAAGAGGTGG-3'

Other clones with same target sequence:

(none)