Construct: shRNA TRCN0000181603
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_198647.1-3037s1c1
- DNA Barcode:
- GCCTTTGTCTTCTCAGTGTAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Tbc1d22b (381085)
- Gene Description:
- TBC1 domain family, member 22B
- Transcript:
- RefSeq NM_198647.1 (CURRENT)
- Match location:
- Position 3037 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000181603)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTTTGTCTTCTCAGTGTAA
- Hairpin Sequence:
-
5'-CCGG-GCCTTTGTCTTCTCAGTGTAA-CTCGAG-TTACACTGAGAAGACAAAGGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTTTGTCTTCTCAGTGTAACTCGAGTTACACTGAGAAGACAAAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTTTGTCTTCTCAGTGTAACTCGAGTTACACTGAGAAGACAAAGGC-3'
Other clones with same target sequence:
(none)
