Construct: shRNA TRCN0000181736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173375.1-602s1c1
DNA Barcode:
GATAACATTAACCCTGGCGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam180a (208164)
Gene Description:
family with sequence similarity 180, member A
Transcript:
RefSeq NM_173375.1 (CURRENT)
Match location:
Position 602 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000181736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAACATTAACCCTGGCGTA
Hairpin Sequence:
5'-CCGG-GATAACATTAACCCTGGCGTA-CTCGAG-TACGCCAGGGTTAATGTTATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAACATTAACCCTGGCGTACTCGAGTACGCCAGGGTTAATGTTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAACATTAACCCTGGCGTACTCGAGTACGCCAGGGTTAATGTTATC-3'

Other clones with same target sequence:

(none)