Construct: shRNA TRCN0000182003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198037.1-4490s1c1
DNA Barcode:
CCTGATGAACTGACCTGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cachd1 (320508)
Gene Description:
cache domain containing 1
Transcript:
RefSeq NM_198037.1 (CURRENT)
Match location:
Position 4490 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000182003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGAACTGACCTGACAT
Hairpin Sequence:
5'-CCGG-CCTGATGAACTGACCTGACAT-CTCGAG-ATGTCAGGTCAGTTCATCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGAACTGACCTGACATCTCGAGATGTCAGGTCAGTTCATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGAACTGACCTGACATCTCGAGATGTCAGGTCAGTTCATCAGG-3'

Other clones with same target sequence:

(none)