Construct: shRNA TRCN0000183619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001011874.1-954s1c1
DNA Barcode:
GCACACAATATACTTAGGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xkr4 (497097)
Gene Description:
X-linked Kx blood group related 4
Transcript:
RefSeq NM_001011874.1 (CURRENT)
Match location:
Position 954 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACAATATACTTAGGTAT
Hairpin Sequence:
5'-CCGG-GCACACAATATACTTAGGTAT-CTCGAG-ATACCTAAGTATATTGTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACAATATACTTAGGTATCTCGAGATACCTAAGTATATTGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACAATATACTTAGGTATCTCGAGATACCTAAGTATATTGTGTGC-3'

Other clones with same target sequence:

(none)