Construct: shRNA TRCN0000184641

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153390.1-585s1c1
DNA Barcode:
CCACCTGTTCCTGGTCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pxt1 (69307)
Gene Description:
peroxisomal, testis specific 1
Transcript:
RefSeq NM_153390.1 (CURRENT)
Match location:
Position 585 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184641)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTGTTCCTGGTCTATTT
Hairpin Sequence:
5'-CCGG-CCACCTGTTCCTGGTCTATTT-CTCGAG-AAATAGACCAGGAACAGGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTGTTCCTGGTCTATTTCTCGAGAAATAGACCAGGAACAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTGTTCCTGGTCTATTTCTCGAGAAATAGACCAGGAACAGGTGG-3'

Other clones with same target sequence:

(none)