Construct: shRNA TRCN0000186749

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146680.1-71s1c1
DNA Barcode:
GCCTGGTCTTATGTCTTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr1423 (258675)
Gene Description:
olfactory receptor 1423
Transcript:
RefSeq NM_146680.1 (CURRENT)
Match location:
Position 71 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000186749)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGTCTTATGTCTTGTTT
Hairpin Sequence:
5'-CCGG-GCCTGGTCTTATGTCTTGTTT-CTCGAG-AAACAAGACATAAGACCAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGTCTTATGTCTTGTTTCTCGAGAAACAAGACATAAGACCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGTCTTATGTCTTGTTTCTCGAGAAACAAGACATAAGACCAGGC-3'

Other clones with same target sequence:

(none)