Construct: shRNA TRCN0000187257

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172194.1-126s1c1
DNA Barcode:
CCTCTTGATAGTGGTAACAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR4Q3 (441669)
Gene Description:
olfactory receptor family 4 subfamily Q member 3
Transcript:
RefSeq NM_172194.1 (CURRENT)
Match location:
Position 126 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000187257)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTGATAGTGGTAACAGT
Hairpin Sequence:
5'-CCGG-CCTCTTGATAGTGGTAACAGT-CTCGAG-ACTGTTACCACTATCAAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTGATAGTGGTAACAGTCTCGAGACTGTTACCACTATCAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTGATAGTGGTAACAGTCTCGAGACTGTTACCACTATCAAGAGG-3'

Other clones with same target sequence:

(none)