Construct: shRNA TRCN0000187286

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019473.1-316s1c1
DNA Barcode:
CGTGCAGATGTTTCTCTCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr155 (29845)
Gene Description:
olfactory receptor 155
Transcript:
RefSeq NM_019473.1 (CURRENT)
Match location:
Position 316 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000187286)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCAGATGTTTCTCTCCTT
Hairpin Sequence:
5'-CCGG-CGTGCAGATGTTTCTCTCCTT-CTCGAG-AAGGAGAGAAACATCTGCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCAGATGTTTCTCTCCTTCTCGAGAAGGAGAGAAACATCTGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCAGATGTTTCTCTCCTTCTCGAGAAGGAGAGAAACATCTGCACG-3'

Other clones with same target sequence:

(none)