Construct: shRNA TRCN0000190557

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013816.1-1314s1c1
DNA Barcode:
GCTGTAAGTCTGTGCTCCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5622 (434459)
Gene Description:
predicted gene 5622
Transcript:
RefSeq NM_001013816.1 (CURRENT)
Match location:
Position 1314 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190557)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTAAGTCTGTGCTCCTAA
Hairpin Sequence:
5'-CCGG-GCTGTAAGTCTGTGCTCCTAA-CTCGAG-TTAGGAGCACAGACTTACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTAAGTCTGTGCTCCTAACTCGAGTTAGGAGCACAGACTTACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTAAGTCTGTGCTCCTAACTCGAGTTAGGAGCACAGACTTACAGC-3'

Other clones with same target sequence:

(none)