Construct: shRNA TRCN0000190631

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173014.1-1573s1c1
DNA Barcode:
GCCCTAAAGCACCCAGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lpcat2 (270084)
Gene Description:
lysophosphatidylcholine acyltransferase 2
Transcript:
RefSeq NM_173014.1 (CURRENT)
Match location:
Position 1573 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190631)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTAAAGCACCCAGAATAT
Hairpin Sequence:
5'-CCGG-GCCCTAAAGCACCCAGAATAT-CTCGAG-ATATTCTGGGTGCTTTAGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTAAAGCACCCAGAATATCTCGAGATATTCTGGGTGCTTTAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTAAAGCACCCAGAATATCTCGAGATATTCTGGGTGCTTTAGGGC-3'

Other clones with same target sequence:

(none)