Construct: shRNA TRCN0000193686

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198425.2-1015s1c1
DNA Barcode:
CGTGGCTTCGTTAATAAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eid2 (386655)
Gene Description:
EP300 interacting inhibitor of differentiation 2
Transcript:
RefSeq NM_198425.2 (CURRENT)
Match location:
Position 1015 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193686)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGCTTCGTTAATAAGTAA
Hairpin Sequence:
5'-CCGG-CGTGGCTTCGTTAATAAGTAA-CTCGAG-TTACTTATTAACGAAGCCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGCTTCGTTAATAAGTAACTCGAGTTACTTATTAACGAAGCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGCTTCGTTAATAAGTAACTCGAGTTACTTATTAACGAAGCCACG-3'

Other clones with same target sequence:

(none)