Construct: shRNA TRCN0000193720

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022331.1-350s1c1
DNA Barcode:
CAATGTGAAGAATCCCTCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Herpud1 (64209)
Gene Description:
homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
Transcript:
RefSeq NM_022331.1 (CURRENT)
Match location:
Position 350 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193720)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATGTGAAGAATCCCTCCAA
Hairpin Sequence:
5'-CCGG-CAATGTGAAGAATCCCTCCAA-CTCGAG-TTGGAGGGATTCTTCACATTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATGTGAAGAATCCCTCCAACTCGAGTTGGAGGGATTCTTCACATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATGTGAAGAATCCCTCCAACTCGAGTTGGAGGGATTCTTCACATTG-3'

Other clones with same target sequence:

(none)