Construct: shRNA TRCN0000194158
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_022331.1-729s1c1
- DNA Barcode:
- GCTCCTATACACAACCAGTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Herpud1 (64209)
- Gene Description:
- homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
- Transcript:
- RefSeq NM_022331.1 (CURRENT)
- Match location:
- Position 729 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000194158)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCCTATACACAACCAGTTT
- Hairpin Sequence:
-
5'-CCGG-GCTCCTATACACAACCAGTTT-CTCGAG-AAACTGGTTGTGTATAGGAGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCCTATACACAACCAGTTTCTCGAGAAACTGGTTGTGTATAGGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCCTATACACAACCAGTTTCTCGAGAAACTGGTTGTGTATAGGAGC-3'
Other clones with same target sequence:
-
TRCN0000297670
