Construct: shRNA TRCN0000194632

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_206868.1-42s1c1
DNA Barcode:
GACTCTTCAAATCCTCCTGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r178 (232959)
Gene Description:
vomeronasal 1 receptor 178
Transcript:
RefSeq NM_206868.1 (CURRENT)
Match location:
Position 42 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194632)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCTTCAAATCCTCCTGCT
Hairpin Sequence:
5'-CCGG-GACTCTTCAAATCCTCCTGCT-CTCGAG-AGCAGGAGGATTTGAAGAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCTTCAAATCCTCCTGCTCTCGAGAGCAGGAGGATTTGAAGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCTTCAAATCCTCCTGCTCTCGAGAGCAGGAGGATTTGAAGAGTC-3'

Other clones with same target sequence:

(none)