Construct: shRNA TRCN0000194810

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006258.1-2589s1c1
DNA Barcode:
CCGGTGTATATACCATACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKG1 (5592)
Gene Description:
protein kinase cGMP-dependent 1
Transcript:
RefSeq NM_006258.1 (NON-CURRENT)
Match location:
Position 2589 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194810)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGTGTATATACCATACAAA
Hairpin Sequence:
5'-CCGG-CCGGTGTATATACCATACAAA-CTCGAG-TTTGTATGGTATATACACCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGTGTATATACCATACAAACTCGAGTTTGTATGGTATATACACCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGTGTATATACCATACAAACTCGAGTTTGTATGGTATATACACCGG-3'

Other clones with same target sequence:

(none)