Construct: shRNA TRCN0000195724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007181.4-286s1c1
DNA Barcode:
CAGAAGGAAATCCTCATATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP4K1 (11184)
Gene Description:
mitogen-activated protein kinase kinase kinase kinase 1
Transcript:
RefSeq NM_007181.4 (NON-CURRENT)
Match location:
Position 286 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAAGGAAATCCTCATATTG
Hairpin Sequence:
5'-CCGG-CAGAAGGAAATCCTCATATTG-CTCGAG-CAATATGAGGATTTCCTTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAAGGAAATCCTCATATTGCTCGAGCAATATGAGGATTTCCTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAAGGAAATCCTCATATTGCTCGAGCAATATGAGGATTTCCTTCTG-3'

Other clones with same target sequence:

(none)