Construct: shRNA TRCN0000195841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172698.2-634s1c1
DNA Barcode:
CCATCGGAGAAAGAAGACCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efcab14 (230648)
Gene Description:
EF-hand calcium binding domain 14
Transcript:
RefSeq NM_172698.2 (CURRENT)
Match location:
Position 634 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCGGAGAAAGAAGACCAA
Hairpin Sequence:
5'-CCGG-CCATCGGAGAAAGAAGACCAA-CTCGAG-TTGGTCTTCTTTCTCCGATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCGGAGAAAGAAGACCAACTCGAGTTGGTCTTCTTTCTCCGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCGGAGAAAGAAGACCAACTCGAGTTGGTCTTCTTTCTCCGATGG-3'

Other clones with same target sequence:

(none)