Construct: shRNA TRCN0000195883

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130870.1-170s1c1
DNA Barcode:
CTTTGGATATGGCTCTGGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krtap19-2 (170651)
Gene Description:
keratin associated protein 19-2
Transcript:
RefSeq NM_130870.1 (CURRENT)
Match location:
Position 170 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195883)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGGATATGGCTCTGGCTA
Hairpin Sequence:
5'-CCGG-CTTTGGATATGGCTCTGGCTA-CTCGAG-TAGCCAGAGCCATATCCAAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGGATATGGCTCTGGCTACTCGAGTAGCCAGAGCCATATCCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGGATATGGCTCTGGCTACTCGAGTAGCCAGAGCCATATCCAAAG-3'

Other clones with same target sequence:

(none)