Construct: shRNA TRCN0000198426

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004180.1-2567s1c1
DNA Barcode:
GCATTTACAAAGCTCTGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam222a (433940)
Gene Description:
family with sequence similarity 222, member A
Transcript:
RefSeq NM_001004180.1 (CURRENT)
Match location:
Position 2567 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198426)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTTACAAAGCTCTGAGAA
Hairpin Sequence:
5'-CCGG-GCATTTACAAAGCTCTGAGAA-CTCGAG-TTCTCAGAGCTTTGTAAATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTTACAAAGCTCTGAGAACTCGAGTTCTCAGAGCTTTGTAAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTTACAAAGCTCTGAGAACTCGAGTTCTCAGAGCTTTGTAAATGC-3'

Other clones with same target sequence:

(none)