Construct: shRNA TRCN0000199721

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173492.1-927s1c1
DNA Barcode:
GCAGTCCCGTTTGACGGTGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP5KL1 (138429)
Gene Description:
phosphatidylinositol-4-phosphate 5-kinase like 1
Transcript:
RefSeq NM_173492.1 (CURRENT)
Match location:
Position 927 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199721)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCCCGTTTGACGGTGGT
Hairpin Sequence:
5'-CCGG-GCAGTCCCGTTTGACGGTGGT-CTCGAG-ACCACCGTCAAACGGGACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCCCGTTTGACGGTGGTCTCGAGACCACCGTCAAACGGGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCCCGTTTGACGGTGGTCTCGAGACCACCGTCAAACGGGACTGC-3'

Other clones with same target sequence:

(none)