Construct: shRNA TRCN0000200331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030259.1-611s1c1
DNA Barcode:
GTCTACTTCCACCCAGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rilpl2 (80291)
Gene Description:
Rab interacting lysosomal protein-like 2
Transcript:
RefSeq NM_030259.1 (CURRENT)
Match location:
Position 611 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTACTTCCACCCAGAGAAA
Hairpin Sequence:
5'-CCGG-GTCTACTTCCACCCAGAGAAA-CTCGAG-TTTCTCTGGGTGGAAGTAGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTACTTCCACCCAGAGAAACTCGAGTTTCTCTGGGTGGAAGTAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTACTTCCACCCAGAGAAACTCGAGTTTCTCTGGGTGGAAGTAGAC-3'

Other clones with same target sequence:

  1. TRCN0000279305