Construct: shRNA TRCN0000202487

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028852.1-452s1c1
DNA Barcode:
GTCTGGCCAAATACCCTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spatc1 (74281)
Gene Description:
spermatogenesis and centriole associated 1
Transcript:
RefSeq NM_028852.1 (CURRENT)
Match location:
Position 452 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202487)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGGCCAAATACCCTTGTT
Hairpin Sequence:
5'-CCGG-GTCTGGCCAAATACCCTTGTT-CTCGAG-AACAAGGGTATTTGGCCAGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGGCCAAATACCCTTGTTCTCGAGAACAAGGGTATTTGGCCAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGGCCAAATACCCTTGTTCTCGAGAACAAGGGTATTTGGCCAGAC-3'

Other clones with same target sequence:

(none)