Construct: shRNA TRCN0000202617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146924.1-70s1c1
DNA Barcode:
CGTGTCATCCTATTTATGGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr476 (258926)
Gene Description:
olfactory receptor 476
Transcript:
RefSeq NM_146924.1 (CURRENT)
Match location:
Position 70 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTCATCCTATTTATGGTA
Hairpin Sequence:
5'-CCGG-CGTGTCATCCTATTTATGGTA-CTCGAG-TACCATAAATAGGATGACACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTCATCCTATTTATGGTACTCGAGTACCATAAATAGGATGACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTCATCCTATTTATGGTACTCGAGTACCATAAATAGGATGACACG-3'

Other clones with same target sequence:

(none)