Construct: shRNA TRCN0000203313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146335.1-538s1c1
DNA Barcode:
GAGCTTAATCAACTGGTACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr19 (18316)
Gene Description:
olfactory receptor 19
Transcript:
RefSeq NM_146335.1 (CURRENT)
Match location:
Position 538 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000203313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTTAATCAACTGGTACTA
Hairpin Sequence:
5'-CCGG-GAGCTTAATCAACTGGTACTA-CTCGAG-TAGTACCAGTTGATTAAGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTTAATCAACTGGTACTACTCGAGTAGTACCAGTTGATTAAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTTAATCAACTGGTACTACTCGAGTAGTACCAGTTGATTAAGCTC-3'

Other clones with same target sequence:

(none)