Construct: shRNA TRCN0000204810

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146826.1-783s1c1
DNA Barcode:
GCCATCTTCGGTCAACTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr969 (258823)
Gene Description:
olfactory receptor 969
Transcript:
RefSeq NM_146826.1 (CURRENT)
Match location:
Position 783 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000204810)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCTTCGGTCAACTCTAT
Hairpin Sequence:
5'-CCGG-GCCATCTTCGGTCAACTCTAT-CTCGAG-ATAGAGTTGACCGAAGATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCTTCGGTCAACTCTATCTCGAGATAGAGTTGACCGAAGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCTTCGGTCAACTCTATCTCGAGATAGAGTTGACCGAAGATGGC-3'

Other clones with same target sequence:

(none)