Construct: shRNA TRCN0000215337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025696.3-3394s1c1
DNA Barcode:
CACTATTTAATGCGCTTAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sorcs3 (66673)
Gene Description:
sortilin-related VPS10 domain containing receptor 3
Transcript:
RefSeq NM_025696.3 (CURRENT)
Match location:
Position 3394 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTATTTAATGCGCTTAATC
Hairpin Sequence:
5'-CCGG-CACTATTTAATGCGCTTAATC-CTCGAG-GATTAAGCGCATTAAATAGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTATTTAATGCGCTTAATCCTCGAGGATTAAGCGCATTAAATAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTATTTAATGCGCTTAATCCTCGAGGATTAAGCGCATTAAATAGTG-3'

Other clones with same target sequence:

(none)